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人白介素2酶免試劑盒,(IL-2)ELISA檢測試劑盒 ,英文名:IL-2 ELISA Kit ,英文縮寫:IL-2 ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白介素2ELISA檢測試劑盒,IL-2 檢測試劑盒, IL-2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人白介素2酶免試劑盒,(IL-2)ELISA檢測試劑盒 操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復性。
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雞熱休克蛋白70(HSP-70)ELISA試劑盒 Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit Y0301096T/48T
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雞白介素18(IL-18)ELISA試劑盒 Chicken Interleukin 18,IL-18 ELISA KitY0301496T/48T
雞白介素1(IL-1)ELISA試劑盒 Chicken Interleukin 1,IL-1 ELISA KitY0301596T/48T
雞病毒性腸炎病毒(DEV)ELISA試劑盒 Chicken virus enteritis,DEV ELISA KitY0301696T/48T
雞肌紅蛋白(MYO/MB) ELISA試劑盒Chicken Myoglobin,MYO/MB ELISA Kit Y0301796T/48T
雞白介素9(IL-9)ELISA試劑盒Chicken Interleukin 9,IL-9 ELISA Kit Y0301896T/48T
雞白介素3(IL-3)ELISA試劑盒Chicken Interleukin 3,IL-3 ELISA Kit Y0301996T/48T
雞白血病抑制因子(LIF)ELISA試劑盒 Chicken Leukemia inhibitory factor,LIF ELISA Kit Y0302096T/48T
雞轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒 Chicken Transforming Growth factor β1,TGF-β1 ELISA Kit Y0302196T/48T
雞C反應(yīng)蛋白(CRP)ELISA試劑盒 Chicken C-Reactive Protein,CRP ELISA Kit Y0302296T/48T
雞血管內(nèi)皮細胞生長因子(VEGF)ELISA試劑盒 Chicken Vascular Endothelial cell Growth Factor,VEGF ELISA Kit Y0302396T/48T
雞免疫球蛋白G(IgG)ELISA試劑盒 Chicken Immunoglobulin G,IgG ELISA Kit Y0302496T/48T
雞堿性成纖維細胞生長因子(bFGF)ELISA試劑盒 Chicken basic fibroblast growth factor,bFGF ELISA Kit Y0302596T/48T
雞可溶性細胞間粘附分子1(sICAM-1)ELISA試劑盒Chicken Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit Y0302696T/48T
雞可溶性E選擇素(sE-selectin)ELISA試劑盒 Chicken soluble E-selectin,sE-selectin ELISA Kit Y0302796T/48T
雞表皮生長因子(EGF)ELISA試劑盒 Chicken Epidermal growth factor,EGF ELISA Kit Y0302896T/48T
雞胰島素樣生長因子1(IGF-1)ELISA試劑盒 Chicken Insulin-like growth factors 1,IGF-1 ELISA Kit Y0302996T/48T
雞生長激素(GH)ELISA試劑盒 Chicken growth hormone,GH ELISA Kit Y0303096T/48T
雞乙酰乙酸檢測(ACAC)ELISA試劑盒Chicken Acetoacetic acid,ACAC ELISA Kit Y0303196T/48T
雞傳染性鼻炎抗體(IC)ELISA試劑盒Chicken infectious coryza,ic ELISA KitY0303296T/48T
雞透明質(zhì)酸(HA)ELISA試劑盒Chicken Hyaluronic acid,HA ELISA KitY0303396T/48T
雞催乳素(PRL)ELISA試劑盒Chicken prolactin,PRL ELISA KitY0303496T/48T
雞白血病病毒抗原(ALV-AG)ELISA試劑盒Chicken Avian Leukosis Virus AG,ALV-AG ELISA KitY0303596T/48T
雞促卵泡素(FSH)ELISA試劑盒Chicken follicle-stimulating hormone,FSH ELISA Kit Y0303696T/48T
雞促黃體激素(LH)ELISA試劑盒Chicken Luteinizing Hormone,LH ELISA KitY0303796T/48T
雞γ干擾素(IFN-γ)ELISA試劑盒 Chicken Interferon γ ,IFN-γ ELISA Kit Y0303896T/48T
雞白介素6(IL-6)ELISA試劑盒Chicken Interleukin 6,IL-6 ELISA KitY0303996T/48T
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雞白介素2(IL-2)ELISA試劑盒Chicken Interleukin 2,IL-2 ELISA KitY0304696T/48T
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雞腎上腺髓質(zhì)素(ADM)ELISA試劑盒 Chicken adrenomedullin,ADM ELISA KitY0305096T/48T
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。