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附睪蛋白酶抑制蛋白
產品時間:2016-11-08
附睪蛋白酶抑制蛋白,公司抗體來源:Rabbit 、Mouse 、Goat 、Rat、Sheep、Chicken、Hamster 、Guinea Pig 、Donkey、Human 、Armenian Hamster、Pig 、Escherichia Coli (E. coli) 、Duck等物種

本產品僅用于科研,不用于臨床

英文名稱 Anti-Eppin
中文名稱 附睪蛋白酶抑制蛋白
別 名 SPINLW1; WAP7; WFDC7; Variant; Cancer/testis antigen 71; CT71; Epididymal protease inhibitor; Protease inhibitor WAP7; Serine protease inhibitor-like with Kunitz and WAP domains 1; EPPI_MOUSE; WAPfour-disulfide core domain protein 7.
濃 度 1mg/1ml
說 明 書 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Mouse, Rat
產品類型 一抗
研究領域 腫瘤 細胞生物 免疫學 發(fā)育生物學 生長因子和激素 轉錄調節(jié)因子 泛素
蛋白分子量 predicted molecular weight: 13kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from mouse Eppin
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產品應用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and omeric. This gene is a member of the WFDC gene family and belongs to the omeric cluster. The protein can inhibit human sperm motility, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. [provided by RefSeq].
Function : Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility (By similarity).
Subunit : Monomer (By similarity). Homodimer (By similarity). Homomultimers (By similarity). Interacts (via C-terminus) with SEMG1 (via 164-283 AA) (By similarity). Interacts with LTF (By similarity). Found in a complex with LTF, CLU, EPPIN and SEMG1 (By similarity).
Subcellular Location : Secreted. Cell surface. Note=Present on the surface of spermatozoa both before and after capacitation.
Tissue Specificity : Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium.
Similarity : Contains 1 BPTI/Kunitz inhibitor domain.
Contains 1 WAP domain.
Database links : UniProtKB/Swiss-Prot: Q9DA01.1
附睪蛋白酶抑制蛋白(Eppin)是一種睪丸/附睪特異性蛋白,可能與精子在附睪的成熟密切相關.
宿主:Human、Murine、Rat (Rattus)、Bovine、Dog (Canine)等物種
應用范圍: WB, ELISA, IP, IHC (p), IHC (f), IF
緩沖液:0.01m PBS,pH值7.4和10毫克/毫升BSA
儲存液:0.1%(w/v)*

 

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