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人整合SV40基因的乳腺上皮細胞;HBL-100 [HBL100]
上皮細胞樣
貼壁生長
該細胞由E.V. Gaffney及其同事從一位沒有乳癌家族史的供者乳汁中建立,人整合SV40基因的乳腺上皮細胞;HBL-100 [HBL100]培養(yǎng)出來的細胞染色體組型在第7代時就不正常;電鏡照片顯示有微絲、張力原纖維和橋粒;Southern轉移表明有整合型SV40病毒基因,不可以當作正常細胞。
DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
C4
基礎培養(yǎng)基+8%DMSO+20%FBS
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